alpha 2 Antiplasmin antibody (Internal Region)

Catalog No. ABIN334343

The Goat Polyclonal anti-alpha 2 Antiplasmin antibody has been validated for WB, IHC, ELISA, IF and FACS. It is suitable to detect alpha 2 Antiplasmin in samples from Human. There is 1 publication available.

Quick Overview for alpha 2 Antiplasmin antibody (Internal Region) (ABIN334343)

Target: alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This alpha 2 Antiplasmin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-alpha 2 Antiplasmin Antibody

Binding Specificity: Internal Region

Purpose: Alpha2-antiplasmin

Sequence: KDFLQSLKGF PRGDK

Specificity: This antibody is expected to recognise both reported isoforms (NP_000925.2, NP_001159393.1). Reported variants represent identical protein (NP_000925.2, NP_001159392.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KDFLQSLKGFPRGDK, from the internal region of the protein sequence according to NP_000925.2, NP_001159393.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Liver shows spotty cytoplasm staining in hepatocytes. Recommended concentration: 3-5 μg/mL.

Western Blot: Approx 70 kDa band observed in lysates of cell line HepG2 (calculated MW of 54.6 kDa according to NP_000925.2). The observed molecular weight is observed by other sources. Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at r

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.<

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-alpha 2 Antiplasmin antibody (ABIN334343)

Abdul, Dekkers, Ariëns, Leebeek, Rijken, Uitte de Willige: "On the localization of the cleavage site in human alpha-2-antiplasmin, involved in the generation of the non-plasminogen binding form." in: Journal of thrombosis and haemostasis : JTH, Vol. 18, Issue 5, pp. 1162-1170, (2020) (PubMed).

Target Details for alpha 2 Antiplasmin

Target: alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))

Alternative Name: SERPINF2

Background: SERPINF2, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2, A2AP, AAP, ALPHA-2-PI, API, PLI, alpha-2-plasmin inhibitor, serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment

Gene ID: 5345

NCBI Accession: NP_000925, NP_001159393

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones