CXCR3 antibody (C-Term)

Catalog No. ABIN334381

This Goat Polyclonal antibody specifically detects CXCR3 in FACS, ELISA, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for CXCR3 antibody (C-Term) (ABIN334381)

Target: CXCR3 (Chemokine (C-X-C Motif) Receptor 3 (CXCR3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CXCR3 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Verified

Product Details anti-CXCR3 Antibody

Binding Specificity: C-Term

Purpose: CXCR3 / GPR9

Sequence: RRDSSWSETS EA

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RRDSSWSETSEA, from the C Terminus of the protein sequence according to NP_001495.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 6-8 μg/mL.

Western Blot: Preliminary experiments showed a band at approx 55-60 kDa in Human Tonsil and Mouse Spleen lysates after 1 μg/mL antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at r

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the membranes of A431 and HepG2 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CXCR3

Target: CXCR3 (Chemokine (C-X-C Motif) Receptor 3 (CXCR3))

Alternative Name: CXCR3

Background: CXCR3, chemokine (C-X-C motif) receptor 3, HGNC:4540, CD183, CKR-L2, CMKAR3, GPR9, IP10, IP10-R, Mig-R, MigR, G protein-coupled receptor 9, IP10 receptor, Mig receptor, chemokine (C-X-C) receptor 3

Gene ID: 2833, 12766, 84475

NCBI Accession: NP_001495, NP_001136269