DACH1 antibody (Internal Region)

Catalog No. ABIN334382

The Goat Polyclonal anti-DACH1 antibody has been validated for WB and ELISA. It is suitable to detect DACH1 in samples from Human.

Quick Overview for DACH1 antibody (Internal Region) (ABIN334382)

Target: DACH1 (Dachshund 1 (DACH1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DACH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-DACH1 Antibody

Binding Specificity: Internal Region

Purpose: Dachshund homolog 1

Sequence: SPVENTPQNN ECK

Specificity: This antibody is expected to recognise all three reported isoforms(NP_542937.1, NP_542938.1, NP_004383.2).

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence SPVENTPQNNECK, from the internal region of the protein sequence according to NP_542937.1, NP_542938.1, NP_004383.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 75 and 52 kDa bands observed in Human Kidney lysates (calculated MW of 73.0 kDa according to NP_542937.1 and of 51.9 kDa according to NP_004383.2). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DACH1

Target: DACH1 (Dachshund 1 (DACH1))

Alternative Name: DACH1

Background: OTTHUMP00000040839, dachshund homolog 1 (Drosophila) , DACH1, RP11-512J14.1, DACH, FLJ10138, dachshund homolog 1

Gene ID: 1602, 13134

NCBI Accession: NP_542937, NP_542938, NP_004383

Pathways: Feeding Behaviour