GAD antibody (Internal Region)

Catalog No. ABIN334405

This Goat Polyclonal antibody specifically detects GAD in WB, ELISA, IF and FACS. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for GAD antibody (Internal Region) (ABIN334405)

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GAD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-GAD Antibody

Binding Specificity: Internal Region

Purpose: GAD1 (isoform GAD67)

Sequence: PDSPQRREKL HK

Specificity: This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PDSPQRREKLHK, from the internal region of the protein sequence according to NP_000808.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 65-70 kDa band observed in Human Cerebral Cortex lysates (calculated MW of 66.9 kDa according to NP_000808.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-GAD antibody (ABIN334405)

Magri, Giacopuzzi, La Via, Bonini, Ravasio, Elhussiny, Orizio, Gangemi, Valsecchi, Bresciani, Barbon, Vita, Gennarelli: "A novel homozygous mutation in GAD1 gene described in a schizophrenic patient impairs activity and dimerization of GAD67 enzyme." in: Scientific reports, Vol. 8, Issue 1, pp. 15470, (2018) (PubMed).

Target Details for GAD

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Alternative Name: GAD1

Background: GAD1, glutamate decarboxylase 1 (brain, 67 kDa), FLJ45882, GAD, SCP, OTTHUMP00000041055, glutamate decarboxylase 1, glutamate decarboxylase 1 (brain, 67kD), GAD67, GAD25

Gene ID: 2571, 14415, 24379

NCBI Accession: NP_000808