HEXIM1 antibody (C-Term)

Catalog No. ABIN334418

The Goat Polyclonal anti-HEXIM1 antibody has been validated for WB, ELISA and IF. It is suitable to detect HEXIM1 in samples from Human and Mouse. There are 2+ publications available.

Quick Overview for HEXIM1 antibody (C-Term) (ABIN334418)

Target: HEXIM1 (Hexamethylene Bis-Acetamide Inducible 1 (HEXIM1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This HEXIM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-HEXIM1 Antibody

Binding Specificity: C-Term

Purpose: HEXIM1

Sequence: HRQQERAPLS KFGD

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HRQQERAPLSKFGD, from the C Terminus of the protein sequence according to NP_006451.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 40 kDa band observed in Human and Mouse Heart lysates (calculated MW of 40.6 kDa according to Human NP_006451.1 and 40.2 kDa according to Mouse NP_620092.1. Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room tem

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nucleus of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-HEXIM1 antibody (ABIN334418)

Bugai, Quaresma, Friedel, Lenasi, Düster, Sibley, Fujinaga, Kukanja, Hennig, Blasius, Geyer, Ule, Dölken, Barborič: "P-TEFb Activation by RBM7 Shapes a Pro-survival Transcriptional Response to Genotoxic Stress." in: Molecular cell, Vol. 74, Issue 2, pp. 254-267.e10, (2019) (PubMed).

Faust, Li, Bacon, Jang, Weiss, Jayaraman, Newton, Krogan, DOrso, Frankel: "The HIV-1 Tat protein recruits a ubiquitin ligase to reorganize the 7SK snRNP for transcriptional activation." in: eLife, Vol. 7, (2019) (PubMed).

Target Details for HEXIM1

Target: HEXIM1 (Hexamethylene Bis-Acetamide Inducible 1 (HEXIM1))

Alternative Name: HEXIM1

Background: HEXIM1, hexamethylene bis-acetamide inducible 1, HGNC:24953, CLP1, HIS1, MAQ1 , HMBA-inducible, hexamethylene bisacetamide-inducible protein, hexamethylene-bis-acetamide-inducible transcript 1, hexamthylene bis-acetamide inducible 1, menage a quatre 1, ED

Gene ID: 10614, 192231

NCBI Accession: NP_006451