HTATSF1 antibody (Internal Region)

Catalog No. ABIN334420

This Goat Polyclonal antibody specifically detects HTATSF1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for HTATSF1 antibody (Internal Region) (ABIN334420)

Target: HTATSF1 (HIV-1 Tat Specific Factor 1 (HTATSF1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HTATSF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-HTATSF1 Antibody

Binding Specificity: Internal Region

Purpose: HTATSF1

Sequence: QELYGDGKDG DTQTD

Cross-Reactivity: Dog, Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QELYGDGKDGDTQTD, from the internal region (near the N Terminus) of the protein sequence according to NP_055315.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 140 kDa band observed in lysates of cell line Daudi (calculated MW of 85.9 kDa according to NP_055315.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhou and Sharp, Science.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HTATSF1

Target: HTATSF1 (HIV-1 Tat Specific Factor 1 (HTATSF1))

Alternative Name: HTATSF1

Background: HTATSF1, HIV-1 Tat specific factor 1 , RP1-196E23.2, TAT-SF1, dJ196E23.2, HIV TAT specific factor 1, cofactor required for Tat activation of HIV-1 transcription

Gene ID: 27336, 317612

NCBI Accession: NP_055315