IKZF1 antibody (Internal Region)

Catalog No. ABIN334424

The Goat Polyclonal anti-IKZF1 antibody has been validated for WB and ELISA. It is suitable to detect IKZF1 in samples from Human.

Quick Overview for IKZF1 antibody (Internal Region) (ABIN334424)

Target: IKZF1 (IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IKZF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-IKZF1 Antibody

Binding Specificity: Internal Region

Purpose: IKZF1 / IKAROS

Sequence: DLLRAASENS QDALR

Specificity: This antibody is expected to recognise all reported human isoforms.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DLLRAASENSQDALR, from the internal region of the protein sequence according to NP_006051.1, NP_001207694.1, NP_001207695.1, NP_001207696.1, NP_001207697.1, NP_001207698.1, NP_001207699.1, NP_001207700.1 and more.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 65 kDa band observed in lysates of cell line MOLT4 (calculated MW of 57.5 kDa according to NP_006051.1). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IKZF1

Target: IKZF1 (IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1))

Alternative Name: IKZF1

Background: IKZF1, IKAROS family zinc finger 1 (Ikaros) , Hs.54452, IK1, IKAROS, LYF1, PRO0758, ZNFN1A1, hIk-1 , CLL-associated antigen KW-6, Ikaros (zinc finger protein), zinc finger protein, subfamily 1A, 1 (Ikaros)

Gene ID: 10320, 22778

NCBI Accession: NP_006051, NP_001207694, NP_001207695, NP_001207696, NP_001207697, NP_001207698, NP_001207699

Pathways: Production of Molecular Mediator of Immune Response