NOVA1 antibody (Internal Region)

Catalog No. ABIN334449

The Goat Polyclonal anti-NOVA1 antibody (ABIN334449) specifically detects NOVA1 in WB, ELISA and IHC. The antibody is reactive with Human samples.

Quick Overview for NOVA1 antibody (Internal Region) (ABIN334449)

Target: NOVA1 (Neuro-Oncological Ventral Antigen 1 (NOVA1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NOVA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-NOVA1 Antibody

Binding Specificity: Internal Region

Purpose: NOVA1

Sequence: REMPQNVAKT EPVS

Specificity: This antibody is expected to recognise all three reported isoforms (NP_002506.2 , NP_006480.2 , NP_006482.1)

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-REMPQNVAKTEPVS, from the internal region of the protein sequence according to NP_002506.2 , NP_006480.2 , NP_006482.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex) and Heart. Recommended concentration: 5 μg/mL.

Western Blot: Approx 50-55 kDa band observed in Human Breast Cancer lysates (calculated MW of 51.7 kDa according to NP_002506.2). In transfected HEK293 transiently expressing NOVA1 a band of approx. 52 kDa is observed. This band is not observed in the non-t

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NOVA1

Target: NOVA1 (Neuro-Oncological Ventral Antigen 1 (NOVA1))

Alternative Name: NOVA1

Background: NOVA1, neuro-oncological ventral antigen 1, Nova-1, paraneoplastic Ri antigen, ventral neuron-specific protein 1

Gene ID: 4857

NCBI Accession: NP_002506, NP_006480, NP_006482