ORAI1 antibody (Internal Region)

Catalog No. ABIN334452

The Goat Polyclonal anti-ORAI1 antibody (ABIN334452) specifically detects ORAI1 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for ORAI1 antibody (Internal Region) (ABIN334452)

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ORAI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-ORAI1 Antibody

Binding Specificity: Internal Region

Purpose: ORAI1 / CRACM1

Sequence: KKQPGQPRPT SKP

Cross-Reactivity: Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KKQPGQPRPTSKP, from the internal region of the protein sequence according to NP_116179.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 50-55 kDa band observed in Human Placenta and Skin lysates (calculated MW of 32.7 kDa according to NP_116179.2). This molecular weight is routinely observed by other sources e.g. Li et al, PMID: 17702753. Recommended concentration: 0

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ORAI1

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Alternative Name: ORAI1

Background: ORAI1, CRACM1, ORAI calcium release-activated calcium modulator 1, FLJ14466, ORAT1, TMEM142A, calcium release-activated calcium channel protein 1, calcium release-activated calcium modulator 1, transmembrane protein 142A

Gene ID: 84876, 109305

NCBI Accession: NP_116179

Pathways: TCR Signaling, BCR Signaling