TFEC antibody (Internal Region)

Catalog No. ABIN334506

This anti-TFEC antibody is a Goat Polyclonal antibody detecting TFEC in ELISA and IHC. Suitable for Human and Mouse.

Quick Overview for TFEC antibody (Internal Region) (ABIN334506)

Target: TFEC (Transcription Factor EC (TFEC))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This TFEC antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-TFEC Antibody

Binding Specificity: Internal Region

Purpose: Tcfec (mouse)

Sequence: HITKQQTHPE RN

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HITKQQTHPERN, from the internal region of the protein sequence according to NP_112475.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Mouse Thymus shows nuclear staining in sporadic cells of the medula. Recommended concentration: 4-6 μg/mL. Paraffin embedded Human Kidney and Spleen. Recommended concentration: 5 μg/mL.

Western Blot: Preliminary experiments gave one band at approx 37 kDa and much stronger bands at approx 70+28 kDa in Mouse Testis lysates after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the s

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TFEC

Target: TFEC (Transcription Factor EC (TFEC))

Alternative Name: Tcfec

Background: Tcfec, transcription factor EC , BB107417, MGC107474, TFEC

Gene ID: 21426, 26296

NCBI Accession: NP_112475