THRA antibody (Internal Region)

Catalog No. ABIN334507

This Goat Polyclonal antibody specifically detects THRA in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for THRA antibody (Internal Region) (ABIN334507)

Target: THRA (Thyroid Hormone Receptor, alpha (THRA))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This THRA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-THRA Antibody

Binding Specificity: Internal Region

Purpose: THRA

Sequence: KEEMIRSLQQ RP

Specificity: This antibody is expected to recognize both reported isoforms (NP_955366.1, NP_003241.2). This antibody is not expected to cross-react to THRB.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KEEMIRSLQQRP, from the internal region of the protein sequence according to NP_955366.1, NP_003241.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil and Breast. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 60+50 kDa bands observed in Human Bone Marrow lysates (calculated MW of 54.8 kDa according to NP_003241.2 and of 46.8 kDa according to NP_955366.1). Recommended concentration: 0.5-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for THRA

Target: THRA (Thyroid Hormone Receptor, alpha (THRA))

Alternative Name: THRA

Background: THRA, thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian), AR7, EAR7, ERB-T-1, ERBA, ERBA1, MGC000261, MGC43240, NR1A1, THRA1, THRA2, c-ERBA-1, ERBA-related 7, avian erythroblastic leukemia viral (v-erb-a) onc

Gene ID: 7067, 21833, 81812

NCBI Accession: NP_955366, NP_003241

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Sensory Perception of Sound, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Maintenance of Protein Location, Skeletal Muscle Fiber Development