TPD52L2 antibody (C-Term)

Catalog No. ABIN334516

This Goat Polyclonal antibody specifically detects TPD52L2 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for TPD52L2 antibody (C-Term) (ABIN334516)

Target: TPD52L2 (Tumor Protein D52-Like 2 (TPD52L2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TPD52L2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-TPD52L2 Antibody

Binding Specificity: C-Term

Purpose: TPD52L2 / D54

Sequence: SGDKPLSDPA P

Specificity: This antibody is expected to recognise all reported isoforms (NP_955392.1, NP_955393.1, NP_955394.1, NP_955395.1, NP_003279.2, NP_955391.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SGDKPLSDPAP, from the C Terminus of the protein sequence according to NP_955392.1, NP_955393.1, NP_955394.1, NP_955395.1, NP_003279.2, NP_955391.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen, Tonsil and Brain (Cortex). Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 28 kDa band observed in Human Breast Cancer and Human Brain lysates (calculated MW of 24.9 kDa according to NP_955392.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. See also our D52 and D53 products.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TPD52L2

Target: TPD52L2 (Tumor Protein D52-Like 2 (TPD52L2))

Alternative Name: TPD52L2

Background: TPD52L2, D54, tumor protein D52-like 2 , DKFZp686A1765, hD54, HCCR-binding protein 2

Gene ID: 7165

NCBI Accession: NP_955392, NP_955393, NP_955394, NP_955395, NP_003279, NP_955391