USP28 antibody (Internal Region)

Catalog No. ABIN334522

This Goat Polyclonal antibody specifically detects USP28 in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for USP28 antibody (Internal Region) (ABIN334522)

Target: USP28 (Ubiquitin Specific Peptidase 28 (USP28))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This USP28 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-USP28 Antibody

Binding Specificity: Internal Region

Purpose: USP28

Sequence: SVELKHYIQE DN

Cross-Reactivity: Cow, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SVELKHYIQEDN, from the internal region of the protein sequence according to NP_065937.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Testis shows acrosomal staining in spermatids. Recommended concentration, 2.5-3.8 μg/mL.

Western Blot: Preliminary experiments gave bands at approx 150 kDa and 50 kDa in Human Bone Marrow lysates after 0.2 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calcu

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for USP28

Target: USP28 (Ubiquitin Specific Peptidase 28 (USP28))

Alternative Name: USP28

Background: USP28, ubiquitin specific peptidase 28, KIAA1515, ubiquitin specific protease 28

Gene ID: 57646

NCBI Accession: NP_065937