COXII antibody

Catalog No. ABIN334535

The Rabbit Polyclonal anti-COXII antibody has been validated for WB and BN PAGE. It is suitable to detect COXII in samples from Arabidopsis thaliana, Moss, Plantago euryphylla, Plantago major, Silene dioica, Silene uniflora, Wild Cabbage, Chickpea, Nicotiana tabacum and Zea mays. There are 4+ publications available.

Quick Overview for COXII antibody (ABIN334535)

Target: COXII (Cytochrome Oxidase Subunit II (COXII))

Reactivity: Arabidopsis thaliana, Moss, Plantago euryphylla, Plantago major, Silene dioica, Silene uniflora, Wild Cabbage, Chickpea, Nicotiana tabacum, Zea mays

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COXII antibody is un-conjugated

Application: Western Blotting (WB), Blue-native PAGE (BN PAGE)

Product Details anti-COXII Antibody

Specificity: Arabidopsis thaliana (leaf extract and isolated mitochondria).

Predicted Reactivity: dicots including Cucumis melo, Glycine max, Pisum sativum, monocots including Oryza sativa, moss Physcomitrella patens

Characteristics: Expected / apparent Molecular Weight of the Antigene: 29.4 / 30 kDa (for Arabidopsis thaliana)

Purification: serum

Immunogen: KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots includingArabidopsis thaliana AtmG00160, monocots including Oryza sativa P04373 and Physcomitrella patens Q1XGA9

Application Details

Application Notes: Recommended Dilution 1 : 1000 (WB), 1 : 1000 (BN-PAGE).

Comment:

antibody detects COXII protein in total leaf extracts and isolated mitochondriaBlue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS pH 7.4

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-COXII antibody (ABIN334535)

Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

Wang, Vanlerberghe: "A lack of mitochondrial alternative oxidase compromises capacity to recover from severe drought stress." in: Physiologia plantarum, (2013) (PubMed).

Hopff, Wienkoop, Lüthje: "The plasma membrane proteome of maize roots grown under low and high iron conditions." in: Journal of proteomics, Vol. 91, pp. 605-18, (2013) (PubMed).

Miller, Grant, Giles, Ribas-Carbo, Berry, Watling, Robinson: "In the heat of the night - alternative pathway respiration drives thermogenesis in Philodendron bipinnatifidum." in: The New phytologist, (2010) (PubMed).

Target Details for COXII

Target: COXII (Cytochrome Oxidase Subunit II (COXII))

Alternative Name: COXII

Background: AGI Code: ATMG00160
Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Alternative name: cytochrome c oxidase subunit 2

Molecular Weight: expected: 29.4 kDa, apparent: 30 kDa (for Arabidopsis thaliana)

UniProt: Q1XGA9, P04373