Cytochrome Oxidase Subunit II (COXII) antibody

Details for Product No. ABIN93555
Antigen
Reactivity
Arabidopsis thaliana, Moss (Physcomitrella patens), Plantago euryphylla, Plantago major, Silene dioica, Silene uniflora, Wild cabbage (Brassica oleracea)
2
2
2
2
2
2
1
1
1
1
1
1
Host
Chicken
3
Clonality
Polyclonal
Application
Western Blotting (WB)
3
1
Options
Immunogen KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots includingArabidopsis thaliana AtmG00160, monocots including Oryza sativa P04373 and Physcomitrella patens Q1XGA9
Isotype IgY
Specificity Arabidopsis thaliana (leaf extract and isolated mitochondria).
Cross-Reactivity (Details) Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
Predicted Reactivity eudicots, monocots
Characteristics Expected / apparent Molecular Weight of the Antigene: 29.4 / 30 kDa (for Arabidopsis thaliana)
Purification affinity purified
Antigen
Alternative Name COXII
Background AGI Code: ATMG00160
Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
Molecular Weight expected: 29.4 kDa, apparent: 30 kDa (for Arabidopsis thaliana)
UniProt P04373, Q1XGA9
Application Notes 1:1000 with standard ECL (WB)
Restrictions For Research Use only
Format Liquid
Buffer PBS pH 8.0+ 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Make aliquots to avoid repreated freeze-thaw cycles and working with a stock.
Storage 4 °C
Storage Comment store at 4°C, make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Product cited in: Yoshida, Watanabe, Hachiya, Tholen, Shibata, Terashima, Noguchi: "Distinct responses of the mitochondrial respiratory chain to long- and short-term high-light environments in Arabidopsis thaliana." in: Plant, cell & environment, 2011 (PubMed).

Moellering, Benning: "Phosphate regulation of lipid biosynthesis in Arabidopsis is independent of the mitochondrial outer membrane DGS1 complex." in: Plant physiology, Vol. 152, Issue 4, pp. 1951-9, 2010 (PubMed).

Grant, Miller, Watling, Robinson: "Synchronicity of thermogenic activity, alternative pathway respiratory flux, AOX protein content, and carbohydrates in receptacle tissues of sacred lotus during floral development." in: Journal of experimental botany, Vol. 59, Issue 3, pp. 705-14, 2008 (PubMed).

Leroch, Neuhaus, Kirchberger, Zimmermann, Melzer, Gerhold, Tjaden: "Identification of a novel adenine nucleotide transporter in the endoplasmic reticulum of Arabidopsis." in: The Plant cell, Vol. 20, Issue 2, pp. 438-51, 2008 (PubMed).