TOP2 antibody (C-Term)

Catalog No. ABIN334538

There is 1 publication for this product available. The Rabbit Polyclonal anti-TOP2 antibody is suitable to detect TOP2 in samples from Arabidopsis thaliana and Vicia faba. It has been validated for WB and IL.

Quick Overview for TOP2 antibody (C-Term) (ABIN334538)

Target: TOP2 (DNA Topoisomerase II (TOP2))

Reactivity: Arabidopsis thaliana, Vicia faba

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunolocalization (IL)

Product Details anti-TOP2 Antibody

Binding Specificity: C-Term

Purpose: Anti-TOP2 | DNA topoisomerase II

Specificity: Antibody detects a protein of ca. 170 kDa on western blots of Arabidopsis thaliana protein extracts. In subcellular fractions of cultured Arabidopsis cells the antibody detects a 170 kDa protein exclusively in the nulear fraction.

Cross-Reactivity (Details): Arabidopsis thaliana, Vicia faba
Not reactive in: Nicotiana tabacum

Predicted Reactivity: Brassica rapa,Chlamydomonas reinhardtii,Chlorella vulgaris,Citrus clementina,Glycine max,Hordeum vulgare,Medicago truncatula,Oryza sativa,Ostreococcus tauri,Panicum italicum,Phaseolus vulgaris,Physcomitrium patens,Pinus sitcHensis,Populus trichocarpa,Solanum tuberosum,Sorghum bicolor,Triticum aestivum,Vitis vinifera,Volvox Caterii

Purification: Serum

Immunogen: The C-terminal 153 amino acids of the Arabidopsis thaliana Topoisomerase II (At3g23890

Application Details

Application Notes: 1 : 500 (IL), 1 : 2000 (WB)

Comment:

Topoisomerase II is highly expressed in young seedlings. The protein is localized in the nucleus and gene expression levels are increased in proliferative tissues like shoot apex or root tip.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-TOP2 antibody (ABIN334538)

Xie, Lam: "Characterization of a DNA Topoisomerase II cDNA from Arabidopsis thaliana." in: Plant physiology, Vol. 106, Issue 4, pp. 1701-2, (1995) (PubMed).

Target Details for TOP2

Target: TOP2 (DNA Topoisomerase II (TOP2))

Alternative Name: TOP2

Background:

Background: Topoisomerase type II (EC5.99.1.3) is one of the enzymes which is catalyzing unknotting of DNA by creating transient breaks in the DNA using a conserved tyrosine as the catalytic residue.Synonyme names of this protein: At3g23890, ATTOPII, DNA topoisomerase 2, DNA topoisomerase II, F14O13.7, TOP2, TOPOISOMERASE II

Additional information: Antibody detects a protein of ca. 170 kDa on western blots of Arabidopsis thaliana protein extracts. In subcellular fractions of cultured Arabidopsis thaliana cells the antibody detects a 170 kDa protein exclusively in the nulear fraction (see picture).

Molecular Weight: 164 | 170 kDa (Arabidopsis thaliana)

Gene ID: 821972

UniProt: P30182

Pathways: Mitotic G1-G1/S Phases