TOP2 antibody (C-Term, N-Term)

Catalog No. ABIN334538

The Rabbit Polyclonal anti-TOP2 antibody has been validated for WB and IL. It is suitable to detect TOP2 in samples from Arabidopsis thaliana and Vicia faba. There is 1 publication available.

Quick Overview for TOP2 antibody (C-Term, N-Term) (ABIN334538)

Target: TOP2 (DNA Topoisomerase II (TOP2))

Reactivity: Arabidopsis thaliana, Vicia faba

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunolocalization (IL)

Product Details anti-TOP2 Antibody

Binding Specificity: C-Term, N-Term

Specificity: Antibody detects a protein of ca. 170 kDa on western blots of Arabidopsis thaliana protein extracts. In subcellular fractions of cultured Arabidopsis cells the antibody detects a 170 kDa protein exclusively in the nulear fraction.

Cross-Reactivity (Details): Not reactive in: Nicotiana tabacum

Predicted Reactivity: Brassica rapa, Chlamydomonas reinhardtii, Chlorella vulgaris, Citrus clementina, Glycine max, Hordeum vulgare, Medicago truncatula, Oryza sativa, Ostreococcus tauri, Panicum italicum, Phaseolus vulgaris, Physcomitrella patens, Pinus sitchensis, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Vitis vinifera, Volvox caterii

Characteristics: Expected / apparent Molecular Weight of the Antigene: 164 / 170 kDa (Arabidopsis thaliana)

Purification: serum

Immunogen: The C-terminal 153 amino acids of the Arabidopsis thaliana Topoisomerase II (At3g23890, protein accesion number P30182) with an N-terminal hexahistidine tag was expressed in E.coli and purified by Ni2+ affinity chromatography.

Application Details

Application Notes: 1: 2000 (WB), 1: 500 (IL)

Comment:

Topoisomerase II is highly expressed in young seedlings. The protein is localized in the nucleus and gene expression levels are increased in proliferative tissues like shoot apex or root tip.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References for anti-TOP2 antibody (ABIN334538)

Xie, Lam: "Characterization of a DNA Topoisomerase II cDNA from Arabidopsis thaliana." in: Plant physiology, Vol. 106, Issue 4, pp. 1701-2, (1995) (PubMed).

Target Details for TOP2

Target: TOP2 (DNA Topoisomerase II (TOP2))

Alternative Name: TOP2

Background: AGI Code: At3g23890
Topoisomerase type II (EC5.99.1.3) is one of the enzymes which is catalyzing unknotting of DNA by creating transient breaks in the DNA using a conserved tyrosine as the catalytic residue.Synonyme names of this protein: At3g23890, ATTOPII, DNA topoisomerase 2, DNA topoisomerase II, F14O13.7, TOP2, TOPOISOMERASE II

Molecular Weight: expected: 164 kDa, apparent: 170 kDa (Arabidopsis thaliana)

UniProt: P30182

Pathways: Mitotic G1-G1/S Phases