Ferrritin 2 antibody

Catalog No. ABIN334549

This Rabbit Polyclonal antibody specifically detects Ferrritin 2 in WB. It exhibits reactivity toward Chlamydomonas reinhardtiiand has been mentioned in 1 publication.

Quick Overview for Ferrritin 2 antibody (ABIN334549)

Target: Ferrritin 2 (Fer2)

Reactivity: Chlamydomonas reinhardtii

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details anti-Ferrritin 2 Antibody

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: Chlamydomonas reinhardtii

Characteristics: Expected / apparent Molecular Weight of the Antigene: 32 kDa (with transitpeptide), 16 (monomer) and aprox. 60 kDa (trimer) for Chlamydomonas reinhardtii

Purification: serum

Immunogen: KLH-conjugated synthetic peptide derived from Fer2 protein sequence (A8WCT5) from Chlamydomonas reinhardtii

Application Details

Application Notes: 0.7361111111

Comment:

a picture with a western blot application example can be found in the reference given below

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References for anti-Ferrritin 2 antibody (ABIN334549)

Long, Sommer, Allen, Lu, Merchant: "FER1 and FER2 encoding two ferritin complexes in Chlamydomonas reinhardtii chloroplasts are regulated by iron." in: Genetics, Vol. 179, Issue 1, pp. 137-47, (2008) (PubMed).

Target Details for Ferrritin 2

Target: Ferrritin 2 (Fer2)

Background: Ferritin is the major iron storage protein complex in eukaryotic cells, consisting of 24 ferritin subunit polypeptides. In Chlamydomonas reinhardtii 2 isoforms, ferritin1 (Fer1) and ferritin2 (Fer2) are present. Both are plastid localized but Fer1 is quantitatively recovered in soluble extracts of cells while Fer2 is found in the particulate fraction. Partial purification of the Fer1 complex indicates that the two ferritins are associated in distinct complexes and do not coassemble. The ratio of Fer1 to Fer2 in iron-replete cells is 70:1 and indicates a more dominant role of Fer1 in iron homeostasis.

Molecular Weight: expected: 32 (with transitpeptide) , apparent: 16 (monomer) and ~60 (trimer) for Chlamydomonas reinhardtii

UniProt: A8WCT5