Netrin 1 antibody (C-Term)

Catalog No. ABIN335150

The Goat Polyclonal anti-Netrin 1 antibody has been validated for ELISA and IF. It is suitable to detect Netrin 1 in samples from Human. There are 2+ publications available.

Quick Overview for Netrin 1 antibody (C-Term) (ABIN335150)

Target: Netrin 1 (NTN1)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Netrin 1 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-Netrin 1 Antibody

Binding Specificity: C-Term

Purpose: Netrin 1

Sequence: KFQQREKKGK CKKA

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence KFQQREKKGKCKKA, from the C Terminus of the protein sequence according to NP_004813.2.

Isotype: IgG

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the membrane and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Netrin 1 antibody (ABIN335150)

Jayakumar, Nauta, Bakker, Bilo, Gansevoort, Johnson, Ramesh: "Netrin-1, a urinary proximal tubular injury marker, is elevated early in the time course of human diabetes." in: Journal of nephrology, Vol. 27, Issue 2, pp. 151-7, (2014) (PubMed).

White, Mohamed, Jayakumar, Ramesh: "Tubular injury marker netrin-1 is elevated early in experimental diabetes." in: Journal of nephrology, Vol. 26, Issue 6, pp. 1055-64, (2013) (PubMed).

Target Details for Netrin 1

Target: Netrin 1 (NTN1)

Alternative Name: NTN1

Background: Netrin 1, HGNC:8029, NTN1, NTN1L, Netrin-1, netrin 1, mouse, homolog of

Gene ID: 9423, 18208, 114523

NCBI Accession: NP_004813

Pathways: Regulation of Cell Size