Enkephalin antibody (Internal Region)

Catalog No. ABIN335158

This Goat Polyclonal antibody specifically detects Enkephalin in IHC and ELISA. It exhibits reactivity toward Mouse and Rat.

Quick Overview for Enkephalin antibody (Internal Region) (ABIN335158)

Target: Enkephalin (PENK) (Proenkephalin (PENK))

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Enkephalin antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Enkephalin Antibody

Binding Specificity: Internal Region

Purpose: Proenkephalin (mouse)

Sequence: YKDSSKQDES H

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-YKDSSKQDESH, from the internal region of the protein sequence according to NP_001002927.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Frozen section of Rat Brain (Striatum) shows staining of dense enkephalinergic axon plexus. Frozen section of the Mouse Brain (Stria terminalis) shows staining of a dense enkephalinergic fibre network in the bed nucleus. Recommended

Western Blot: Preliminary experiments gave an approx 40 kDa band in Rat Brain lysates after 0.2 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.8 kDa

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Enkephalin

Target: Enkephalin (PENK) (Proenkephalin (PENK))

Alternative Name: PENK

Background: Proenkephalin

Gene ID: 18619, 29237

NCBI Accession: NP_001002927

Pathways: Stem Cell Maintenance