GFAP antibody

Catalog No. ABIN335275

This Rabbit Polyclonal antibody specifically detects GFAP in WB, IHC, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Rat, Mouse and Zebrafish (Danio rerio) and has been mentioned in 2+ publications.

Quick Overview for GFAP antibody (ABIN335275)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: K39

Product Details anti-GFAP Antibody

Specificity: Human, mouse and rat.

Purification: Antiserum

Immunogen: K39 is a rabbit polyclonal antiserum derived by immunization of a rabbit with a glial fibrillary acidic protein preparation from human spinal cord.

Application Details

Application Notes: K39 reacts exclusively with glial fibrillary acidic protein which is present in astrocytes in the central nervous system and Schwann cells. K39 is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C

References for anti-GFAP antibody (ABIN335275)

Herpers, Ramaekers, Aldeweireldt, Moesker, Slooff: "Co-expression of glial fibrillary acidic protein- and vimentin-type intermediate filaments in human astrocytomas." in: Acta neuropathologica, Vol. 70, Issue 3-4, pp. 333-9, (1986) (PubMed).

Ramaekers, Puts, Moesker, Kant, Huysmans, Haag, Jap, Herman, Vooijs: "Antibodies to intermediate filament proteins in the immunohistochemical identification of human tumours: an overview." in: The Histochemical journal, Vol. 15, Issue 7, pp. 691-713, (1983) (PubMed).

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: Glial fibrillary acidic protein / GFAP

Background: GFAP (55 kD) is selectively located in astrocytes and represents the major constituent of astrocytic intermediate filaments. GFAP expression levels are highly variable during development of the central nervous system. In adults, GFAP levels increase as a result of the proliferation of astrocytes that occurs in a response to a variety of physical, chemical and etiological insults, including Alzheimer€™s disease, epilepsy and multiple sclerosis. In the peripheral nervous system GFAP is expressed by Schwann cells. Upon differentiation, myelin forming Schwann cells down-regulate GFAP, whereas in non-myelin forming Schwann cells GFAP persists into adulthood.