Integrin Beta1D antibody

Catalog No. ABIN335351

The Mouse Monoclonal anti-Integrin Beta1D antibody has been validated for IHC, WB, ICC and IHC (fro). It is suitable to detect Integrin Beta1D in samples from Human, Mouse and Dog. There is 1 publication available.

Quick Overview for Integrin Beta1D antibody (ABIN335351)

Target: Integrin Beta1D (LOC395211)

Reactivity: Human, Mouse, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin Beta1D antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2B1

Product Details anti-Integrin Beta1D Antibody

Specificity: Human, mouse and dog. A broad species reactivity is expected because of the conserved nature of the epitope.

Purification: Purified

Immunogen: 2B1 is a mouse monoclonal IgG1, kappa antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a mouse immunized with a synthetic peptide corresponding to the C-terminal 24 amino acids of integrin beta1D including an appending N-terminal cysteine (CQENPIYKS- PINNFKNPNYGRKAGL) coupled to keyhole limpet hemocyanin.

Isotype: IgG1

Application Details

Application Notes: 2B1 recognizes specifically the cytoplasmic domain of integrin subunit beta1D present in cardiac and skeletal muscle. 2B1 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Storage: 4 °C

References for anti-Integrin Beta1D antibody (ABIN335351)

van der Flier, Gaspar, Thorsteinsdóttir, Baudoin, Groeneveld, Mummery, Sonnenberg: "Spatial and temporal expression of the beta1D integrin during mouse development." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 210, Issue 4, pp. 472-86, (1998) (PubMed).

Target Details for Integrin Beta1D

Target: Integrin Beta1D (LOC395211)

Alternative Name: Integrin beta 1D

Background: Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated alpha and beta subunits. More than 18 alpha and 8 beta subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. There are two major forms of integrin beta1: beta1A and beta1D, which differ in 13 amino acids. Their distribution pattern in adult tissues is mutually exclusive. beta1A is present in all tissues, except cardiac and skeletal muscle, which instead express the beta1D variant.