Desmin antibody

Catalog No. ABIN335360

The Mouse Monoclonal anti-Desmin antibody has been validated for WB, IHC and IHC (fro). It is suitable to detect Desmin in samples from Human, Rat, Mouse, Chicken, Hamster, Pig, Dog, Rabbit and Zebrafish (Danio rerio). There are 8+ publications available.

Quick Overview for Desmin antibody (ABIN335360)

Target: Desmin (DES)

Reactivity: Human, Rat, Mouse, Chicken, Hamster, Pig, Dog, Rabbit, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RD301

Product Details anti-Desmin Antibody

Specificity: Human, mouse, rat, rabbit, hamster, canine, chicken and swine.

Purification: Purified

Immunogen: RD301 is a mouse monoclonal IgG2b antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a cytoskeletal desmin extract of chicken gizzard.

Isotype: IgG2b

Application Details

Application Notes: RD301 reacts exclusively with desmin, which is expressed in smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma. RD301 is suitable for immunoblotting and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Storage: 4 °C

References for anti-Desmin antibody (ABIN335360)

Council, Hameed: "Differential expression of immunohistochemical markers in bladder smooth muscle and myofibroblasts, and the potential utility of desmin, smoothelin, and vimentin in staging of bladder carcinoma." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 22, Issue 5, pp. 639-50, (2009) (PubMed).

Dispersyn, Geuens, Ver Donck, Ramaekers, Borgers: "Adult rabbit cardiomyocytes undergo hibernation-like dedifferentiation when co-cultured with cardiac fibroblasts." in: Cardiovascular research, Vol. 51, Issue 2, pp. 230-40, (2001) (PubMed).

Schaart, Pieper, Kuijpers, Bloemendal, Ramaekers: "Baby hamster kidney (BHK-21/C13) cells can express striated muscle type proteins." in: Differentiation; research in biological diversity, Vol. 46, Issue 2, pp. 105-15, (1991) (PubMed).

Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, (1990) (PubMed).

Schaart, Viebahn, Langmann, Ramaekers: "Desmin and titin expression in early postimplantation mouse embryos." in: Development (Cambridge, England), Vol. 107, Issue 3, pp. 585-96, (1990) (PubMed).

Pieper, Schaart, Krimpenfort, Henderik, Moshage, van de Kemp, Ramaekers, Berns, Bloemendal: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, (1989) (PubMed).

Verhagen, Aalders, Ramaekers, Debruyne, Schalken: "Differential expression of keratins in the basal and luminal compartments of rat prostatic epithelium during degeneration and regeneration." in: The Prostate, Vol. 13, Issue 1, pp. 25-38, (1988) (PubMed).

Quax, van den Broek, Egberts, Ramaekers, Bloemendal: "Characterization of the hamster desmin gene: expression and formation of desmin filaments in nonmuscle cells after gene transfer." in: Cell, Vol. 43, Issue 1, pp. 327-38, (1986) (PubMed).

Target Details for Desmin

Target: Desmin (DES)

Alternative Name: Desmin

Background: Desmin is a 53 kDa intermediate filament protein that exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.