Desmin antibody (C-Term)

Catalog No. ABIN334386

Product Details anti-Desmin Antibody

Target: Desmin (DES)

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Desmin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: Desmin

Sequence: RDGEVVSEAT QQQHE

Cross-Reactivity: Cow, Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-RDGEVVSEATQQQHE, from the C Terminus of the protein sequence according to NP_001918.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 4-6 μg/mL.

Western Blot: Approx. 55-60 kDa band observed in Human and Mouse Skeletal Muscle lysates and in Human Heart lysates (calculated MW of 53.5 kDa according to Human NP_001918.3, and Mouse NP_034173.1). Preliminary testing showed a band at approx 60 kDa in Rat

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Desmin antibody (ABIN334386)

Aue, Englert, Harrer, Schwiering, Gaab, König, Adams, Schmidtko, Friebe, Groneberg: "NO-sensitive guanylyl cyclase discriminates pericyte-derived interstitial from intra-alveolar myofibroblasts in murine pulmonary fibrosis." in: Respiratory research, Vol. 24, Issue 1, pp. 167, (2023) (PubMed).

Target Details for Desmin

Target: Desmin (DES)

Alternative Name: DES (DES Products)

Synonyms: DES antibody, des-b antibody, MGC80853 antibody, des antibody, csm1 antibody, csm2 antibody, desm antibody, cmd1i antibody, MGC75911 antibody, LOC100220724 antibody, desmin antibody, cb290 antibody, fb59a12 antibody, wu:fb59a12 antibody, zgc:109859 antibody, CSM1 antibody, CSM2 antibody, LGMD2R antibody, wu:fc11d08 antibody, zgc:154009 antibody, des-a antibody, MGC52614 antibody, desmin antibody, desmin, gene 1 L homeolog antibody, desmin, gene 1 antibody, desmin a antibody, desmin b antibody, desmin, gene 1 S homeolog antibody, desmin, gene 2 S homeolog antibody, DES antibody, des.1.L antibody, des.1 antibody, des antibody, desma antibody, Des antibody, desmb antibody, des.1.S antibody, des.2.S antibody

Background: DES, desmin, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, intermediate filament protein

Gene ID: 1674, 13346, 64362

NCBI Accession: NP_001918