NEFH antibody

Catalog No. ABIN335370

This anti-NEFH antibody is a Mouse Monoclonal antibody detecting NEFH in WB, IHC and IHC (fro). Suitable for Human, Rat, Mouse, Chicken, Cow, Rabbit, Guinea Pig, Dog, Sheep, Xenopus laevis, Monkey, Hamster and Zebrafish (Danio rerio).

Quick Overview for NEFH antibody (ABIN335370)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivity: Human, Rat, Mouse, Chicken, Cow, Rabbit, Guinea Pig, Dog, Sheep, Xenopus laevis, Monkey, Hamster, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEFH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RNF405

Product Details anti-NEFH Antibody

Specificity: Human, rabbit, rat, dog, chicken, mouse, hamster, cow, monkey, guinea pig, sheep and Xenopus.

Purification: The antibody is affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using a non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: RNF405 is a mouse monoclonal IgG2a antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a neurofilament preparation of calf brain tissue.

Isotype: IgG2a

Application Details

Application Notes: RNF405 reacts exclusively with the phosphorylated isoform of the 200 kD neurofilament protein. RNF405 is suitable for immunoblotting and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:50 - 1:100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:500 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Alternative Name: Neurofilament 200 kDa

Background: Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiation.