OB Cadherin antibody

Catalog No. ABIN335403

This anti-OB Cadherin antibody is a Mouse Monoclonal antibody detecting OB Cadherin in WB, IHC, ICC and IHC (fro). Suitable for Human and Rat. This Primary Antibody has been cited in 2+ publications.

Quick Overview for OB Cadherin antibody (ABIN335403)

Target: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This OB Cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 16A

Product Details anti-OB Cadherin Antibody

Specificity: Human and rat.

Purification: Protein A affinity column

Immunogen: 16A is a mouse monoclonal IgG1 antibody obtained by fusion of SP2/0 mouse myeloma cells with spleen cells from a mouse immunized with affinity purified extracellular domain of human cadherin-11-GST fusion protein.

Isotype: IgG1

Application Details

Application Notes: 16A recognizes the extracellular domain of cadherin-11. 16A is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen sections when using a PBS buffer containing 0.1 mM CaCl 2 and 0.1 mM MgCl 2 . Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:50 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:25 - 1:250 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Storage: 4 °C

References for anti-OB Cadherin antibody (ABIN335403)

Tomita, van Bokhoven, van Leenders, Ruijter, Jansen, Bussemakers, Schalken: "Cadherin switching in human prostate cancer progression." in: Cancer research, Vol. 60, Issue 13, pp. 3650-4, (2000) (PubMed).

Bussemakers, Van Bokhoven, Tomita, Jansen, Schalken: "Complex cadherin expression in human prostate cancer cells." in: International journal of cancer. Journal international du cancer, Vol. 85, Issue 3, pp. 446-50, (2000) (PubMed).

Target Details for OB Cadherin

Target: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Alternative Name: OB-Cadherin / Cadherin-11

Background: Cadherins constitute a family of transmembrane glycoproteins involved in Ca 2+ -dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. The progression of carcinomas is associated with the loss of epithelial morphology and a concomitant acquisition of a more mesenchymal phenotype, which is thought to contribute to the invasive and/or metastatic behavior. A putative role for cadherin-11 in these late stages of tumor progression is based on the fact that migration of mesenchymal cells is facilitated when cadherin-11 is highly expressed.