FLIP antibody (N-Term)

Catalog No. ABIN336100

This Mouse Monoclonal antibody specifically detects FLIP in WB and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for FLIP antibody (N-Term) (ABIN336100)

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This FLIP antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 5D8

Product Details anti-FLIP Antibody

Binding Specificity: AA 1-376, N-Term

Specificity: The antibody recognizes FLIP.

Characteristics: Synonyms: c-FLIP, CLARP, MRIT, CASH, FLAME-1, CASP8AP1, Usurpin, CASP8 and FADD-like apoptosisregulator, Caspase-eight-related protein, Inhibitor of FLICE, Cellular FLICE-like inhibitoryprotein, Caspase-like apoptosis regulatory protein, MACH-related inducer of toxicity,Caspase homolog, FADD-like antiapoptotic molecule 1

Purification: Affinity Chromatography on Protein G

Immunogen: Recombinant human FLIP (aa 1-376) purified from E. coli.

Isotype: IgG3

Application Details

Application Notes: ELISA. Western Blot (1/500-1/1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C or -70 °Cfor longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for FLIP

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Alternative Name: CFLAR / Casper / I-FLICE

Background: FLIP, also known as CASP8 and FADD-like apoptosis regulator (CFLAR), contains two death effector domains (DEDs) and a caspase-like domain. FLIP may play a crucial role between cell survival and cell death pathway in mammalian cells and interacts with adapter protein FADD and Caspase-8 and -10, and potently inhibits apoptosis induced by all known death receptors DR3 (Death Receptor 3), TRAIL-R (TNF-related apoptosis-inducing ligand receptor) and TNFR1 (Tumor Necrosis Factor Receptor 1).Synonyms: CASH, CASP8 and FADD-like apoptosis regulator, CASP8AP1, CLARP, Caspase homolog, Caspase-eight-related protein, Caspase-like apoptosis regulatory protein, Cellular FLICE-like inhibitory protein, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, MACH-related inducer of toxicity, MRIT, Usurpin, c-FLIP

Gene ID: 8837

UniProt: O15519

Pathways: Apoptosis, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development