14-3-3 theta antibody (N-Term)

Catalog No. ABIN336110

The Mouse Monoclonal anti-14-3-3 theta antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect 14-3-3 theta in samples from Human.

Quick Overview for 14-3-3 theta antibody (N-Term) (ABIN336110)

Target: 14-3-3 theta (YWHAQ)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This 14-3-3 theta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: AT1A1

Product Details anti-14-3-3 theta Antibody

Binding Specificity: AA 1-245, N-Term

Specificity: The antibody recognizes Human 14-3-3 protein theta (tau). Other species not tested.

Characteristics: Synonyms: 14-3-3 protein tau, 14-3-3 protein T-cell, YWHAQ, Protein HS1

Purification: Protein-G affinity chromatography

Immunogen: Recombinant human 14-3-3 tau (aa 1-245) purified from E. coli

Isotype: IgG1

Application Details

Application Notes: ELISA. Western blot (1/1,000-1/2,000). Immunohistochemistry on Paraffin Sections (10 μg/mL). This YWHAQ antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C or -70 °Cfor longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for 14-3-3 theta

Target: 14-3-3 theta (YWHAQ)

Alternative Name: 14-3-3 Protein theta

Background: The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 tau, a subtype of the 14-3-3 family of proteins, was found in T Cells, brain and testes. This 14-3-3 tau is upregulated in patients with amyotrophic lateral sclerosis.Synonyms: 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1, YWHAQ

Gene ID: 10971

UniProt: P27348

Pathways: Apoptosis, Myometrial Relaxation and Contraction