The Mouse Monoclonal anti-S100A1 antibody has been validated for IHC, IHC (p) and FACS. It is suitable to detect S100A1 in samples from Human, Mouse, Rat, Cow and Monkey.
Catalog No. ABIN336902
Quick Overview for S100A1 antibody (FITC) (ABIN336902)
S100A1
Reactivity: Rat
WB, IHC, ICC, IP
Host: Rabbit
Polyclonal
unconjugated
Application Notes
IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears. For IHC dilute conc. antibodies 1:50-1:100, use streptavidin-biotin system or polymer system, incubate 30 minutes at room temperature. Prediluted antibody is ready to be used for IHC. IF, FITC-conjugated antibodies 10-20 ug/ml (1:10-1:20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4ºC. Flow: FITC-conjugated antibodies, 0.2-1.0 ug/0.1 ml (1:200-1:1,000) (Not tested in our lab). The optimum dilution should be determined by the individual lab. Positive Control: Melanoma or schwannoma. Cellular Localization: Cytoplasmic.
Restrictions
For Research Use only
Format
Liquid
Buffer
The concentrated antibodies are supplied in PBS with 1% BSA, 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color. Description S100 protein is a type of low molecular weight protein fo
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.