Ubiquitin antibody

Catalog No. ABIN336952

This Rabbit Polyclonal antibody specifically detects Ubiquitin in IHC and IHC (p). It exhibits reactivity toward Human, Rat, Mouse, Cow and Horse.

Quick Overview for Ubiquitin antibody (ABIN336952)

Target: Ubiquitin (Ub)

Reactivity: Human, Rat, Mouse, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Ubiquitin Antibody

Specificity: Molecular Weight: 8.5 kDa.

Immunogen: Purified human Ubiquitin

Application Details

Application Notes: IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears) 1:50-1:100 using streptavidin-Biotin system or polymer system. Prediluted antibody is used directly without dilution. FFPE tissue sections require antigen retriever, boiling tissue for 15-20 minutes in 10 mM citrate , pH 6.0, followed by cooling at RT for 15-20 minutes The optimum dilution should be determined by the individual lab. Positive Control: Human brain from Alzheimer’s disease. Cellular Localization: Nuclear, Cytoplasmic, and cell membrane.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is supplied with 1% BSA in PBS with 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquintin

Background: Ubiquitin is a 76 amino acids 8.5 kDa, a highly conserved small regulatory protein that is ubiquitously expressed in eukaryotes and plays a central role in protein degradation. The Ubiquitin/proteosome pathways are main, non-lysomal route for intracellular protein degradation. The protein to be degraded is conjugated to Ubiquitin, it is selectively degraded by 26S complex multicatalytic cytosolic and nuclear protease termed proteosome. This protein also plays important role in cellular processes e.g. Cell cycle progression, gene transcription, antigen processing and signal transduction. In Immunohistochemistry (IHC) this antibody can detect abnormal inclusion bodies, in neurofibrillary tangles in Alzheimer’s disease, lewy bodies in Parkinson’s disease, pick bodies in Pick’s disease, inclusions in motor neuron disease, Mallory’s Haylin in alcoholic liver disease and Rosenthal fibers in astrocytes. This antibody stains senile plaques and neurons.

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway