S1PR3 antibody (AA 12-25)

Catalog No. ABIN337001

The Rabbit Polyclonal anti-S1PR3 antibody has been validated for WB, IHC, IHC (p) and ICC. It is suitable to detect S1PR3 in samples from Human.

Quick Overview for S1PR3 antibody (AA 12-25) (ABIN337001)

Target: S1PR3 (Sphingosine-1-Phosphate Receptor 3 (S1PR3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-S1PR3 Antibody

Binding Specificity: AA 12-25

Brand: IHC-plus™

Specificity: Recognizes human EDG 3 receptor at ~47kD in human cerebral cortex. Liver and heart tissues display 2-3 bands between 40-50kD, possibly due to different degrees of glycosylation.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla (100%) Gibbon (93%) Marmoset (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to aa12-25 [VRGNETLREHYQYV] of human EDG 3 protein. Species sequence Homology: Mouse, rat: 79% (11/14). Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon (93%), Marmoset (86%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IHC, IHC-P (10 μg/mL), WB (3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: TBS,  pH 7.4, 5 mg/mL BSA

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for S1PR3

Target: S1PR3 (Sphingosine-1-Phosphate Receptor 3 (S1PR3))

Alternative Name: SIPR3 / EDG3 / S1P3

Background: Name/Gene ID: S1PR3
Subfamily: Lysophospholipid/Lysosphingolipid
Family: GPCR

Synonyms: S1PR3, EDG-3, EDG3, LPB3, S1P receptor EDG3, S1P3, S1P receptor Edg-3, S1P receptor 3

Gene ID: 1903

UniProt: Q99500