EXOC7 antibody (AA 453-464)

Catalog No. ABIN337025

The Goat Polyclonal anti-EXOC7 antibody is suitable to detect EXOC7 in samples from Human, Mouse, Rat, Cow, Dog, Horse, Pig, Chicken and Monkey. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for EXOC7 antibody (AA 453-464) (ABIN337025)

Target: EXOC7 (Exocyst Complex Component 7 (EXOC7))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Chicken, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This EXOC7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EXOC7 Antibody

Binding Specificity: AA 453-464

Specificity: Human EXOC7. This antibody is expected to recognise all five reported isoforms (NP_001013861.1, NP_056034.2, NP_001138769.1, NP_001138771.1, NP_001138770.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bovine, Elephant, Panda, Horse, Pig, Opossum, Turkey, Chicken, Platypus (100%) Lizard, Salmon, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DNIKNDPDKEYN from an internal region of human EXOC7 (NP_001013861.1, NP_056034.2, NP_001138769.1, NP_001138771.1, NP_001138770.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bovine, Elephant, Panda, Horse, Pig, Opossum, Turkey, Chicken, Platypus (100%), Lizard, Salmon, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:16000), WB (1:16000, 0.5 - 1.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for EXOC7

Target: EXOC7 (Exocyst Complex Component 7 (EXOC7))

Alternative Name: EXOC7

Background: Name/Gene ID: EXOC7

Synonyms: EXOC7, 2-5-3p, Exo70p, EXO70, KIAA1067, YJL085W, EX070, Exocyst complex component 7

Gene ID: 23265

NCBI Accession: NP_001013861, NP_056034, NP_001138769, NP_001138771, NP_001138770

UniProt: Q9UPT5

Pathways: Peptide Hormone Metabolism, ER-Nucleus Signaling, Synaptic Vesicle Exocytosis