OB Cadherin antibody (Intracellular)

Catalog No. ABIN337035

This anti-OB Cadherin antibody is a Mouse Monoclonal antibody detecting OB Cadherin in WB, ELISA, IHC, IP, IHC (fro) and IHC (p). Suitable for Human and Mouse.

Quick Overview for OB Cadherin antibody (Intracellular) (ABIN337035)

Target: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This OB Cadherin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 5B2H5

Product Details anti-OB Cadherin Antibody

Binding Specificity: Intracellular

Brand: IHC-plus™

Specificity: Recognizes the ~110kD human Cadherin 11 protein. Cross-reactivity with other cadherin family members has not been observed.

Purification: Protein A purified

Immunogen: Recombinant protein consisting of intracellular domain of human cadherin 11.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr (5 - 10 μg/mL), IHC-P (5 - 10 μg/mL), IP (5 μg/mL), WB (2 - 4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL. Immunoprecipitation: 5 μg. Native, human, Not tested for mouse. Positive control: MDA. MB231 cells (human) and mouse L fibroblast lysates.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C (no longer than 1 week)
Long term -20°C
Aliquot to avoid freeze/thaw cycles.

Target Details for OB Cadherin

Target: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Alternative Name: CDH11 / Cadherin 11

Background: Name/Gene ID: CDH11
Family: Cadherin

Synonyms: CDH11, CAD11, Cadherin 11, Cadherin-11, CDHOB, OSF-4, Osteoblast cadherin, OB, OB-cadherin, Ventral neural cadherin, Vnc

Gene ID: 1009

UniProt: P55287