OB Cadherin antibody (Intracellular)

Catalog No. ABIN337036

This Rabbit Polyclonal antibody specifically detects OB Cadherin in WB, ELISA, IHC, IHC (fro) and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for OB Cadherin antibody (Intracellular) (ABIN337036)

Target: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OB Cadherin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-OB Cadherin Antibody

Binding Specificity: Intracellular

Brand: IHC-plus™

Specificity: Recognizes human Cadherin 11 at ~110kD. An additional band at ~130kD (presumably the Cadherin 11 precursor) may also be observed. Some crossreactivity with other Cadherins is observed in approximately the following order: Cadherin11>>R-Cadherin>Cadherin 8>>N-Cadherin=P-Cadherin. No crossreactivity with Cadherin 6 or E-Cadherin has been observed.

Purification: Immunoaffinity purified

Immunogen: Recombinant protein derived from the intracellular domain of the human Cadherin 11 protein.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-Fr, IHC-P (5 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Positive control: MDA. MB231 cells (human) and mouse L fibroblast lysates.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for OB Cadherin

Target: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Alternative Name: CDH11 / Cadherin 11

Background: Name/Gene ID: CDH11
Family: Cadherin

Synonyms: CDH11, CAD11, Cadherin 11, Cadherin-11, CDHOB, OSF-4, Osteoblast cadherin, OB, OB-cadherin, Ventral neural cadherin, Vnc

Gene ID: 1009

UniProt: P55287