CKS1 antibody (C-Term)

Catalog No. ABIN337043

The Mouse Monoclonal anti-CKS1 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect CKS1 in samples from Human.

Quick Overview for CKS1 antibody (C-Term) (ABIN337043)

Target: CKS1 (CKS1B) (CDC28 Protein Kinase Regulatory Subunit 1B (CKS1B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CKS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 4G12G7

Product Details anti-CKS1 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Recognizes the 11kD human Cks1 protein. No reactivity was observed with a Cks2 recombinant protein. Cross reactivity with an unknown protein at ~35kD has been observed in Western blots of HeLa cell lysates. Reactivity has been confirmed with a Cks1 recombinant protein as well as HeLa cell lysates. IHC reactivity was confirmed with formalin-fixed, paraffin-embedded (FFPE) human tonsil sections.

Purification: Protein A purified

Immunogen: Synthetic peptide derived from the C-terminal region of the human Cks1 protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for CKS1

Target: CKS1 (CKS1B) (CDC28 Protein Kinase Regulatory Subunit 1B (CKS1B))

Alternative Name: CKS1B / CKS1

Background: Name/Gene ID: CKS1B
Subfamily: CKS

Synonyms: CKS1B, CDC28 protein kinase 1, CKS-1, CDC28 protein kinase 1B, Ckshs1, PNAS-18, PNAS-143, PNAS-16, CDC2-associated protein CKS1, CKS1

Gene ID: 1163

UniProt: P61024

Pathways: Mitotic G1-G1/S Phases, Inositol Metabolic Process