Occludin antibody (C-Term)

Catalog No. ABIN337048

This Mouse Monoclonal antibody specifically detects Occludin in WB, IHC, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Mouse, Rat and Dogand has been mentioned in 1 publication.

Quick Overview for Occludin antibody (C-Term) (ABIN337048)

Target: Occludin (OCLN)

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Occludin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: OC-3F10

Product Details anti-Occludin Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Recognizes mammalian Occludin Protein. Species cross-reactivity: human, mouse, rat and canine.

Purification: Protein A purified

Immunogen: GST fusion protein consisting of the C-terminal region (~150aa) of human occludin.

Type of Immunogen: Fusion protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF (2 - 3 μg/mL), IHC, IHC-P (10 μg/mL), WB (0.1 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Positive control: T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), rat liver and Caco-2 cells (human colon adenocarcinoma). Does not appear to detect the most highly phosphorylated form of the occludin protein when tested on Caco-2 cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

References for anti-Occludin antibody (ABIN337048)

Krueger, Härtig, Reichenbach, Bechmann, Michalski: "Blood-brain barrier breakdown after embolic stroke in rats occurs without ultrastructural evidence for disrupting tight junctions." in: PLoS ONE, Vol. 8, Issue 2, pp. e56419, (2013) (PubMed).

Target Details for Occludin

Target: Occludin (OCLN)

Alternative Name: OCLN / Occludin

Background: Name/Gene ID: OCLN

Synonyms: OCLN, Occludin, BLCPMG

Gene ID: 100506658

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C