Claudin 5 antibody

Catalog No. ABIN337059

This anti-Claudin 5 antibody is a Mouse Monoclonal antibody detecting Claudin 5 in IHC, ELISA, WB and IHC (p). Suitable for Rat and Mouse.

Quick Overview for Claudin 5 antibody (ABIN337059)

Target: Claudin 5 (CLDN5)

Reactivity: Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Claudin 5 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Claudin 5 Antibody

Brand: IHC-plus™

Specificity: Recognizes rat endogenous Claudin-5 protein at ~22-24kD. Species cross-reactivity: WB: mouse, IHC: human.

Purification: Protein A purified

Immunogen: Synthetic peptide derived from the mouse Claudin-5 protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (5 - 10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL. Positive control: Rat lung, kidney, and small intestine homogenates and mouse lung homogenates.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Claudin 5

Target: Claudin 5 (CLDN5)

Alternative Name: CLDN5 / Claudin 5

Background: Name/Gene ID: CLDN5
Family: Claudin

Synonyms: CLDN5, BEC1, AWAL, Claudin-5, CPETRL1, TMDVCF, TMVCF, Claudin 5

Gene ID: 7122

UniProt: O00501

Pathways: Hepatitis C