SRSF1 antibody (N-Term)

Catalog No. ABIN337068

This anti-SRSF1 antibody is a Mouse Monoclonal antibody detecting SRSF1 in WB, IHC, IP, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for SRSF1 antibody (N-Term) (ABIN337068)

Target: SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SRSF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SRSF1 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Recognizes the N-terminal portion (aa1-97) of the SF2/ASF protein comprising the RNA-recognition motif (RRM1). Reactivity is not affected by phosphorylation state of SF2/ASF. Species cross-reactivity: Human (HeLa cells), mouse and rat. Cross-reactivity expected with other species because SF2/ASF is highly conserved.

Purification: Protein A purified

Immunogen: Recombinant SF2/ASF protein.

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Application Details

Application Notes: Approved: IF, IHC, IHC-P (5 μg/mL), IP, WB (0.5 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SRSF1

Target: SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))

Alternative Name: SFRS1 / SF2

Background: Name/Gene ID: SRSF1

Synonyms: SRSF1, Alternative-splicing factor 1, ASF, SF2, SF2p33, SFRS1, Splicing factor 2, ASF-1, SR splicing factor 1, SRp30a

Gene ID: 6426

UniProt: Q07955

Pathways: Ribonucleoprotein Complex Subunit Organization