s100a4 antibody (AA 86-98)

Catalog No. ABIN337109

Product Details anti-s100a4 Antibody

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Binding Specificity: AA 86-98

Reactivity: Human, Cow, Rabbit, Dog, Horse, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This s100a4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human S100A4 / FSP1. Both variants (NP_002952.1, NP_062427.1) represent identical protein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Bovine, Dog, Horse, Rabbit, Pig (100%) Marmoset, Hamster, Elephant, Bat (92%) Mouse, Rat (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide CNEFFEGFPDKQP from the C-terminus of human S100A4 / FSP1 (NP_002952.1, NP_062427.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Bovine, Dog, Horse, Rabbit, Pig (100%), Marmoset, Hamster, Elephant, Bat (92%), Mouse, Rat (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 - 5 μg/mL), Peptide-ELISA (1:128000), WB (0.03 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5-5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for s100a4

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Alternative Name: S100A4 / FSP1 (S100A4 Products)

Synonyms: 18A2 antibody, 42A antibody, CAPL antibody, FSP1 antibody, MTS1 antibody, P9KA antibody, PEL98 antibody, 42a antibody, Capl antibody, FSp1 antibody, Mts1 antibody, PeL98 antibody, metastasin antibody, pk9a antibody, S100A4 antibody, P9ka antibody, RNP9KA antibody, S100 calcium binding protein A4 antibody, S100 calcium-binding protein A4 antibody, S100A4 antibody, S100a4 antibody

Background: Name/Gene ID: S100A4

Synonyms: S100A4, 42A, 18A2, Fibroblast-specific protein-1, FSP1, MTS1, PEL98, Protein Mts1, Calvasculin, CAPL, Metastasin, p9KA, Protein S100-A4

Gene ID: 6275

NCBI Accession: NP_002952, NP_062427

UniProt: P26447

Pathways: S100 Proteins