APOE antibody (AA 305-317)

Catalog No. ABIN337117

This Goat Polyclonal antibody specifically detects APOE in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for APOE antibody (AA 305-317) (ABIN337117)

Target: APOE (Apolipoprotein E (APOE))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-APOE Antibody

Binding Specificity: AA 305-317

Specificity: Human APOE / Apolipoprotein E.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Orangutan, Gibbon (100%) Chimpanzee, Gorilla, Marmoset (92%) Baboon (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-VGTSAAPVPSDNH from the C-terminus of human APOE / Apolipoprotein E (NP_000032.1). Percent identity by BLAST analysis: Human, Orangutan, Gibbon (100%), Chimpanzee, Gorilla, Marmoset (92%), Baboon (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for APOE

Target: APOE (Apolipoprotein E (APOE))

Alternative Name: APOE / Apolipoprotein E

Background: Name/Gene ID: APOE

Synonyms: APOE, AD2, Apo-E, Apolipoprotein E3, Apolipoprotein E, LDLCQ5, LPG

Gene ID: 348

NCBI Accession: NP_000032

UniProt: P02649

Pathways: Regulation of Cell Size, Lipid Metabolism