CBX5 antibody (AA 88-102)

Catalog No. ABIN337121

The Goat Polyclonal anti-CBX5 antibody is suitable to detect CBX5 in samples from Human, Cow, Pig, Horse, Sheep and Monkey. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for CBX5 antibody (AA 88-102) (ABIN337121)

Target: CBX5 (Chromobox Homolog 5 (CBX5))

Reactivity: Human, Cow, Pig, Horse, Sheep, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This CBX5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CBX5 Antibody

Binding Specificity: AA 88-102

Specificity: Human CBX5 / HP1. Variants (NP_036249.1, NP_001120793.1, NP_001120794.1) encode the same protein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Sheep, Bovine, Horse, Pig (100%) Mouse, Rat, Hamster, Elephant, Panda, Dog, Rabbit (93%) Bat (87%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-NKRKSNFSNSADDIK from an internal region of human CBX5 / HP1 (NP_036249.1, NP_001120793.1, NP_001120794.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Sheep, Bovine, Horse, Pig (100%), Mouse, Rat, Hamster, Elephant, Panda, Dog, Rabbit (93%), Bat (87%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:128000), WB (1:128000, 0.03 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CBX5

Target: CBX5 (Chromobox Homolog 5 (CBX5))

Alternative Name: CBX5 / HP1 Alpha

Background: Name/Gene ID: CBX5

Synonyms: CBX5, Chromobox homolog 5, HP1Hs alpha, HP1Hs-alpha, HP1, HP1 alpha homolog, HP1 alpha, HP1-ALPHA, HP1A, Antigen p25, Chromobox protein homolog 5

Gene ID: 23468

NCBI Accession: NP_036249, NP_001120793, NP_001120794

UniProt: P45973