PNPLA2 antibody (AA 68-79)

Catalog No. ABIN337131

The Goat Polyclonal anti-PNPLA2 antibody (ABIN337131) specifically detects PNPLA2 in ELISA, IHC and IHC (p). The antibody is reactive with Human, Mouse, Rat, Cow, Goat, Hamster, Horse and Monkey samples.

Quick Overview for PNPLA2 antibody (AA 68-79) (ABIN337131)

Target: PNPLA2 (Patatin-Like phospholipase Domain Containing 2 (PNPLA2))

Reactivity: Human, Mouse, Rat, Cow, Goat, Hamster, Horse, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This PNPLA2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PNPLA2 Antibody

Binding Specificity: AA 68-79

Specificity: Mouse PNPLA2 / ATGL. Pease note this antibody was designed using the Mouse sequence NP_080078.1, which differs by 2 amino acids from the Human sequence NP_065109.1.

Predicted Reactivity: Percent identity by BLAST analysis: Marmoset, Mouse, Rat, Goat, Hamster, Panda, Bovine, Horse (100%) Dog, Pig, Lizard, Xenopus (92%) Human, Gorilla, Gibbon, Monkey, Elephant, Zebra finch, Chicken, Stickleback, Zebrafish (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-NIIEVSKEARKR from an internal region of mouse PNPLA2 / ATGL (NP_065109.1). Percent identity by BLAST analysis: Marmoset, Mouse, Rat, Goat, Hamster, Panda, Bovine, Horse (100%), Dog, Pig, Lizard, Xenopus (92%), Human, Gorilla, Gibbon, Monkey, Elephant, Zebra finch, Chicken, Stickleback, Zebrafish (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PNPLA2

Target: PNPLA2 (Patatin-Like phospholipase Domain Containing 2 (PNPLA2))

Alternative Name: PNPLA2 / ATGL

Background: Name/Gene ID: PNPLA2

Synonyms: PNPLA2, Adipose triglyceride lipase, ATGL, IPLA2zeta, PEDF-R, IPLA2-zeta, Triglyceride hydrolase, TTS-2.2, TTS2, Transport-secretion protein 2, TTS2.2, Desnutrin, FP17548

Gene ID: 57104

NCBI Accession: NP_065109

UniProt: Q96AD5