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MUC5AC antibody

The Mouse Monoclonal anti-MUC5AC antibody has been validated for IHC, ELISA, IP, ICC, IHC (p) and IHC (fro). It is suitable to detect MUC5AC in samples from Human.
Catalog No. ABIN337160

Quick Overview for MUC5AC antibody (ABIN337160)

Target

See all MUC5AC Antibodies
MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Reactivity

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Human

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This MUC5AC antibody is un-conjugated

Application

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Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone

CLH2
  • Brand

    IHC-plus™

    Specificity

    Recognizes the MUC5AC tandem repeat of human mucin. The predominant staining pattern in normal tissues is perinuclear. Antibody reactivity has been observed in foveolar and mucopeptic neck cells of normal gastric mucosa.

    Purification

    Purified

    Immunogen

    Synthetic peptide of the MUC5AC tandem repeat.

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG1
  • Application Notes

    Approved: ELISA, ICC, IHC, IHC-Fr (1:500 - 1:1000), IHC-P (10 μg/mL), IP

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Positive control: Normal human epithelial tissues: bronchus, endocervix, stomach and gallbladder.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.6, 0.09 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C,-20 °C

    Storage Comment

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Target

    MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

    Alternative Name

    MUC5AC

    Background

    Name/Gene ID: MUC5AC

    Synonyms: MUC5AC, Gastric mucin, Major airway glycoprotein, Muc5/5ac, Mucin-5AC, LeB, Lewis B blood group antigen, Tracheobronchial mucin, TBM, MUC-5AC, MUC5

    Gene ID

    4586

    UniProt

    P98088
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