Pyruvate Kinase, Muscle (PKM) antibody

Catalog No. ABIN337171

This Rabbit Polyclonal antibody specifically detects in WB, IHC, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for Pyruvate Kinase, Muscle (PKM) antibody (ABIN337171)

Target: Pyruvate Kinase, Muscle (PKM)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details

Specificity: Recognizes endogenous levels of total human PKM2 protein at 60kD. Species cross-reactivity: monkey, mouse and rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the sequence of human PKM2 (KLH). Molecular Weight: 60kD.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-Fr (1:100), IHC-P (1:100), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details

Target: Pyruvate Kinase, Muscle (PKM)

Alternative Name: PKM / Pyruvate Kinase, Muscle

Background: Name/Gene ID: PKM

Synonyms: PKM, p58, PK3, PKM2, Pyruvate Kinase, Pyruvate kinase 2/3, Pyruvate kinase, m1, Pyruvate kinase, muscle, TCB, Tumor M2-PK, OIP-3, OIP3, Opa-interacting protein 3, CTHBP, PK, muscle type, PK2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, THBP1

Molecular Weight: 60kDa

Gene ID: 5315

UniProt: P14618

Pathways: Warburg Effect