Cullin 1 antibody (C-Term)

Catalog No. ABIN337174

This Mouse Monoclonal antibody specifically detects Cullin 1 in WB, IHC, ELISA, IP and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Cullin 1 antibody (C-Term) (ABIN337174)

Target: Cullin 1 (CUL1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cullin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cullin 1 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Recognizes Cullin 1. Reacts specifically with the ~86kD CUL-1 protein and does not cross-react with the related CUL-2 protein. This antibody is confirmed to be reactive with human. Reactivity is confirmed from extracts of IMR90 (human fibroblasts) and Wi-38 cells.

Purification: Protein A purified

Immunogen: A synthetic peptide corresponding to an 11 amino acid sequence at the C-terminus of the human CUL-1 protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (10 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Immunoprecipitation: 5-10 μg/ IP reaction.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Cullin 1

Target: Cullin 1 (CUL1)

Alternative Name: Cullin 1 / CUL1

Background: Name/Gene ID: CUL1

Synonyms: CUL1, Cullin 1, Cullin-1, CUL-1

Gene ID: 8454

UniProt: Q13616

Pathways: Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases, Regulation of Cell Size