FAIM antibody (AA 121-132)

Catalog No. ABIN337178

The Goat Polyclonal anti-FAIM antibody is suitable to detect FAIM in samples from Human. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for FAIM antibody (AA 121-132) (ABIN337178)

Target: FAIM (Fas Apoptotic Inhibitory Molecule (FAIM))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FAIM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FAIM Antibody

Binding Specificity: AA 121-132

Specificity: Human FAIM / FAIM1. This antibody is expected to recognise all reported isoforms (NP_001028202.1, NP_001028203.1, NP_001028204.1, NP_060617.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla (100%) Gibbon, Monkey, Marmoset, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus (92%) Mouse, Bat, Rabbit (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KKYMEDRSKTTN from an internal region of human FAIM / FAIM1 (NP_001028202.1, NP_001028203.1, NP_001028204.1, NP_060617.1). Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon, Monkey, Marmoset, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus (92%), Mouse, Bat, Rabbit (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 - 5 μg/mL), Peptide-ELISA (1:32000), WB (0.5 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5-5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for FAIM

Target: FAIM (Fas Apoptotic Inhibitory Molecule (FAIM))

Alternative Name: FAIM

Background: Name/Gene ID: FAIM

Synonyms: FAIM, FAIM1

Gene ID: 55179

NCBI Accession: NP_001028202, NP_001028203, NP_001028204, NP_060617

UniProt: Q9NVQ4