PC antibody (AA 886-899)

Catalog No. ABIN337183

This Goat Polyclonal antibody specifically detects PC in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Pig, Cow, Monkey, Dog, Horse, Hamster, Rabbit and Bat.

Quick Overview for PC antibody (AA 886-899) (ABIN337183)

Target: PC (Pyruvate Carboxylase (PC))

Reactivity: Human, Mouse, Rat, Pig, Cow, Monkey, Dog, Horse, Hamster, Rabbit, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This PC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PC Antibody

Binding Specificity: AA 886-899

Specificity: Human PC / Pyruvate Carboxylase. All reported variants (NP_000911.2, NP_001035806.1, NP_071504.2) represent identical protein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (100%) Goat, Stickleback, Pufferfish (93%) Opossum, Chicken, Xenopus, Zebrafish (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KFKEVKKAYVEANQ from an internal region of human PC / Pyruvate Carboxylase (NP_000911.2, NP_001035806.1, NP_071504.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (100%), Goat, Stickleback, Pufferfish (93%), Opossum, Chicken, Xenopus, Zebrafish (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:16000), WB (0.03 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PC

Target: PC (Pyruvate Carboxylase (PC))

Alternative Name: PC / Pyruvate Carboxylase

Background: Name/Gene ID: PC

Synonyms: PC, PCB, Pyruvate carboxylase, Pyruvic carboxylase

Gene ID: 5091

NCBI Accession: NP_000911, NP_001035806, NP_071504

UniProt: P11498