COPA antibody (AA 1-19)

Catalog No. ABIN337288

This Rabbit Polyclonal antibody specifically detects COPA in WB, IHC, ICC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Horse, Cow, Bat, Chicken, Hamster, Monkey, Pig and Xenopus laevis.

Quick Overview for COPA antibody (AA 1-19) (ABIN337288)

Target: COPA (Coatomer Protein Complex, Subunit alpha (COPA))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Horse, Cow, Bat, Chicken, Hamster, Monkey, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COPA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-COPA Antibody

Binding Specificity: AA 1-19

Brand: IHC-plus™

Specificity: Detects rat coatomer-protein I alpha (COP I alpha). Species cross-reactivity: Human.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bat, Bovine, Dog, Horse, Pig, Opossum, Chicken, Xenopus, Salmon, Stickleback, Pufferfish, Zebrafish, Seq squirt, Ant, Water flea, Tick (100%) Mosquito, Sea anemone, Rice, Beetle, Arabidopsis, Spikemoss (94%) Drosophila, Trichoplax, Grape, Castor bean (89%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide: M(1)LTKFETKSARVKGLSFHP(19)C. Immunizing peptide corresponds to amino acid residues 1-19 from rat COP I alpha. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bat, Bovine, Dog, Horse, Pig, Opossum, Chicken, Xenopus, Salmon, Stickleback, Pufferfish, Zebrafish, Seq squirt, Ant, Water flea, Tick (100%), Mosquito, Sea anemone, Rice, Beetle, Arabidopsis, Spikemoss (94%), Drosophila, Trichoplax, Grape, Castor bean (89%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 1 μg/mL, detects an ~140kD protein representing COPI alpha from PC-12 cell extract. Also detects an unidentified protein at ~100kD.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for COPA

Target: COPA (Coatomer Protein Complex, Subunit alpha (COPA))

Alternative Name: COPA / Xenin

Background: Name/Gene ID: COPA

Synonyms: COPA, Alpha coat protein, Alpha-COP, Alpha COP I, Alpha-coat protein, Coatomer alpha subunit, Coatomer subunit alpha, HEPCOP, HEP-COP, Xenin

Gene ID: 1314

UniProt: P53621

Pathways: Hormone Activity