Thrombospondin 1 antibody (Collagen-binding Domain)

Catalog No. ABIN337292

The Mouse Monoclonal anti-Thrombospondin 1 antibody has been validated for WB, IHC, IP, IF, FACS and IHC (p). It is suitable to detect Thrombospondin 1 in samples from Human, Mouse, Rat, Cow, Horse, Pig, Dog and Sheep.

Quick Overview for Thrombospondin 1 antibody (Collagen-binding Domain) (ABIN337292)

Target: Thrombospondin 1 (THBS1)

Reactivity: Human, Mouse, Rat, Cow, Horse, Pig, Dog, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thrombospondin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: A6-1

Product Details anti-Thrombospondin 1 Antibody

Binding Specificity: Collagen-binding Domain

Brand: IHC-plus™

Specificity: Recognizes Thrombospondin. Shows no cross-reactivity with fibronectin, fibrinogen and von Willebrand factor. Shows a mild cross-reaction with TSP 2. Inhibits TSP-collagen interaction. Antibody binding to TSP is unaffected by glycosaminoglycans (e. g. hyaluronic acid, chondroitin sulfate and heparin). Its binding is enhanced by EDTA i. e. at low concentration of Ca2+. Species cross-reactivity: Human, bovine, porcine, equine, canine, sheep, mouse and rat.

Purification: Protein G purified

Immunogen: Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. MW of Antigen: ~450kD (non-reduced), 170-180kD (reduced). Epitope: Collagen Type V-binding domain of TSP.

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, IF, IHC, IHC-P (10 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4.

Preservative: Azide free

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Thrombospondin 1

Target: Thrombospondin 1 (THBS1)

Alternative Name: Thrombospondin 1

Pathways: Autophagy