C5AR1 antibody

Catalog No. ABIN337372

This Mouse Monoclonal antibody specifically detects C5AR1 in FACS, IHC, IHC (p), Func and Neut. It exhibits reactivity toward Human and Rabbit.

Quick Overview for C5AR1 antibody (ABIN337372)

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Reactivity: Human, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This C5AR1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Neutralization (Neut)

Product Details anti-C5AR1 Antibody

Brand: IHC-plus™

Specificity: Recognizes the C5a receptor (C5aR) CD88, which is predominantly expressed on cells of the myeloid lineage. Clone 3H1738 was raised against a synthetic peptide Met1 -Asn31 and has recently been shown to recognize the heptameric peptide (D15DKDTLD21). Clone 3H1738 inhibits C5a binding to its receptor. Species cross-reactivity: Rabbit.

Purification: Ion exchange chromatography

Immunogen: C5aR - peptide: Met1 - Asn31.

Type of Immunogen: Synthetic peptide

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:50), Func, IHC, IHC-P (10 μg/mL), Neut

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Flow Cytometry: 1:50. Use 20 μL to label 5x10^5 cells in 100 μL. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 40 % glycerol. No preservative added.

Preservative: Azide free

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for C5AR1

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Alternative Name: C5AR1 / CD88 / C5a Receptor

Background: Name/Gene ID: C5AR1
Subfamily: Anaphylatoxin
Family: GPCR

Synonyms: C5AR1, C5A, C5a-R, C5a ligand, C5a receptor, CD88, C5a anaphylatoxin receptor, C5AR, C5R1, CD88 antigen

Gene ID: 728

UniProt: P21730

Pathways: Complement System